Chemically Induced Resistance to Heat Treatment and Stress Protein Synthesis in Cultured Mammalian Cells

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Abstract
Short exposure (1-2h) of cultured cells, derived from a transplantable murine mammary carcinoma, to sodium arsenite, 2,4-dinitrophenol (DNP), carbonylcyanide-3-chlorophenylhydrazone (CCP) or disulfiram, induced resistance to a subsequent heat treatment, similar to heat-induced thermotolerance. Optimum resistance to a test heat treatment of 45 min at 45.degree. C after sodium arsenite exposure was obtained at a concentration of 300 .mu.M, after DNP exposure at 3 mM, after CCP at 300 .mu.M and after disulfiram exposure in the range 1-30 .mu.M. Exposure of cells to CCP, sodium arsenite or disulfiram led to enhanced synthesis of some proteins with the same molecular weight as ''heat shock'' proteins. The pattern of enhanced synthesis of these proteins was agent specific. We could not detect significantly enhanced synthesis of the proteins after DNP using one-dimensional gel electrophoresis. These results suggest that enhanced stress protein synthesis is not a prerequisite for the development of thermal resistance.