Identification of caveolin-1 in lipoprotein particles secreted by exocrine cells
- 16 September 1999
- journal article
- Published by Springer Nature in Nature Cell Biology
- Vol. 1 (6) , 369-375
- https://doi.org/10.1038/14067
Abstract
Caveolin-1 is a protein component (of relative molecular mass 22, 000) of the striated coat that decorates the cytoplasmic surface of caveolae membranes. Previous biochemical and molecular tests have indicated that caveolin-1 is an integral membrane protein that is co-translationally inserted into endoplasmic-reticulum membranes of fibroblast and epithelial cells such that its carboxy- and amino-terminal ends are in the cytoplasm. Here we identify caveolin-1 in the secretory pathway of exocrine cells. Secretion of caveolin-1 from pancreatic acinar cells and a transfected exocrine cell line, but not from Chinese hamster ovary cells, is stimulated by the secretagogues secretin, cholecystokinin and dexamethasone. The secreted caveolin-1 co-fractionates with apolipoproteins, indicating that it may be secreted in a complex with lipids.Keywords
This publication has 30 references indexed in Scilit:
- Molecular Genetics of the Caveolin Gene Family: Implications for Human Cancers, Diabetes, Alzheimer Disease, and Muscular DystrophyAmerican Journal of Human Genetics, 1998
- Baculovirus-based Expression of Mammalian Caveolin in Sf21 Insect CellsPublished by Elsevier ,1996
- Oligomerization of VIP21‐caveolin in vitro is stabilized by long chain fatty acylation or cholesterolFEBS Letters, 1996
- Oligomeric structure of caveolin: implications for caveolae membrane organization.Proceedings of the National Academy of Sciences, 1995
- De novo formation of caveolae in lymphocytes by expression of VIP21-caveolin.Proceedings of the National Academy of Sciences, 1995
- VIP21-caveolin, a membrane protein constituent of the caveolar coat, oligomerizes in vivo and in vitro.Molecular Biology of the Cell, 1995
- Caveolin Is Palmitoylated on Multiple Cysteine ResiduesJournal of Biological Chemistry, 1995
- The sequence of human caveolin reveals identity with VIP21, a component of transport vesiclesFEBS Letters, 1992
- Caveolin, a protein component of caveolae membrane coatsPublished by Elsevier ,1992
- Endothelial plasmalemmal vesicles have a characteristic striped bipolar surface structure.The Journal of cell biology, 1985