Glycogen metabolism: a 13C‐NMR study on the isolated perfused rat heart
Open Access
- 3 December 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 178 (1) , 34-38
- https://doi.org/10.1016/0014-5793(84)81234-x
Abstract
Glycogen synthesis from D‐[1‐13C]glucose was observed in the perfused rat heart by 13C‐NMR spectroscopy at 62.9 MHz. The glycogenogenesis was stimulated by pretreatment of the animals with isoprenaline. Whereas in hearts from control rats the incorporation of D‐[1‐13C]glucose into the glycogen remained below the detection threshold, 5 min proton‐decoupled 13C‐NMR spectra revealed, in hearts from treated rats, a significant labelling of the glycogen within the first minutes of the perfusion and a further linear increase of the glycogen resonance for up to 25 min. This model was used to monitor the appearance of 13C‐labelled lactate during ischemia.Keywords
This publication has 3 references indexed in Scilit:
- In vivo carbon-13 nuclear magnetic resonance studies of heart metabolism.Proceedings of the National Academy of Sciences, 1983
- Structure and metabolism of mammalian liver glycogen monitored by carbon-13 nuclear magnetic resonanceBiochemistry, 1983
- Quantitation of lactic acid in caffeine‐contracted and resting frog muscle by high resolution natural abundance 13C NMRFEBS Letters, 1982