Utilization of Free and Esterified Gholesterol-4-G14 for Corticoid Biosynthesis by Hog Adrenal Homogenates.

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Abstract
Tracer amounts of cholesterol-4-C14 added to hog adrenal homogenates and incubated under conditions favorable for steroid synthesis were rapidly and efficiently converted to labeled steroids. Addition of cholesterol-4-C14 esters, prepared enzymatically and incubated under the same conditions, did not give C14-activity in the steroids produced. Conversion of free cholesterol to cholesterol esters or of esters to free cholesterol as measured by C14-activity of the sterol digitonides did not occur in this system.

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