ULTRAVIOLET ACTION SPECTRUM (238–405 nm) FOR INHIBITION OF GLYCINE UPTAKE IN E. COLI*
- 1 December 1979
- journal article
- research article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 30 (6) , 661-666
- https://doi.org/10.1111/j.1751-1097.1979.tb07196.x
Abstract
Abstract— Initial rate of uptake of 3H‐glycine by Escherichia coli B/r was measured immediately after irradiation with monochromatic light. Uptake was proportional to time for at least 2 min in both control and irradiated samples. Inhibition of uptake is an exponential function of fluence to about 20% remaining activity, beyond which it is much more resistant to irradiation, suggesting two different uptake systems. The principal (sensitive) system shows an F37 of 2.2 kJ/m2 at 280 nm and 110 kJ/m2 at 334 nm. The response is independent of cell killing and of presence of the rel gene. The chromophore remains unidentified, although an action spectrum suggests a protein chromophore in the far‐UV (below 300 nm) region and a menaquinone chromophore in the near‐ultraviolet (above 300 nm).A 10–20%, stimulation of uptake rate, which we cannot account for, is observed at low fluences (generally below 100 kJ/m2) at 313, 366 and 405 nm, but not at 334 nm.This publication has 24 references indexed in Scilit:
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