Selective inhibition of the secondary response of primed cells by incubation with hapten.

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  • 1 September 1968
    • journal article
    • Vol. 15  (3) , 389-94
Abstract
Cells from a mouse immunized with a hapten-carrier conjugate were incubated in vitro with antigen. They gave a secondary antibody response when transferred to syngeneic irradiated mice. The response was partly inhibited if free hapten was added to the incubation mixture. The inhibition was greatest at low antigen concentrations, and in these circumstances production of IgG anti-hapten antibodies was inhibited more than that of IgM antibodies. At nearly all antigen concentrations studied antibodies to the immunizing hapten were inhibited more than those that reacted with a related hapten. With respect to hapten inhibition the characteristics of memory cells resemble those of free antibody.