Identification and partial characterization of parathyroid hormone-related protein in human and bovine milk
- 1 October 1990
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 127 (1) , 167-176
- https://doi.org/10.1677/joe.0.1270167
Abstract
Parathyroid hormone-related protein (PTHrP) was measured in human and bovine milk by radioimmunoassay (RIA) and bioassay, and the molecular forms characterized by gel chromatography and immunoblotting of affinity-purified PTHrP. Mean immunoreactive PTHrP(1–34) concentrations were 23 and 87 μg/l in human and bovine milk respectively. Bioactive (BIO) PTHrP concentrations determined by cyclic AMP production by ROS 17/2·8 cells correlated significantly (P< 0·001) with those obtained by RIA (BIO = 1·04RIA−3·4, r = 0·939). Gel filtration of human and bovine milk identified several peaks with immunoactivity and bioactivity. Immunoblotting of affinity-purified PTHrP revealed multiple molecular species including components with mobilities similar to those of PTHrP and its subfragments. These studies confirm the presence of immuno- and bioactive PTHrP in milk and suggest that post-translational processing is complex and variable. Journal of Endocrinology (1990) 127, 167–176This publication has 20 references indexed in Scilit:
- Two Distinct Tumor-Derived, Parathyroid Hormone-Like Peptides Result from Alternative Ribonucleic Acid SplicingMolecular Endocrinology, 1988
- Expression of a Calcium-Mobilizing Parathyroid Hormone-Like Peptide in Lactating Mammary TissueScience, 1988
- Evidence for a novel parathyroid hormone-related protein in fetal lamb parathyroid glands and sheep placenta: comparisons with a similar protein implicated in humoral hypercalcaemia of malignancyJournal of Endocrinology, 1988
- Parathyroid Hormone-Related Protein of Malignancy: Active Synthetic FragmentsScience, 1987
- Parathyroid hormonelike protein from human renal carcinoma cells. Structural and functional homology with parathyroid hormone.Journal of Clinical Investigation, 1987
- A Parathyroid Hormone-Related Protein Implicated in Malignant Hypercalcemia: Cloning and ExpressionScience, 1987
- Parathyroid hormone-related protein purified from a human lung cancer cell line.Proceedings of the National Academy of Sciences, 1987
- Uptake and transepithelial transport of nerve growth factor in suckling rat ileum.The Journal of cell biology, 1986
- A Parathyroid Hormone-Like Protein from Cultured Human KeratinocytesScience, 1986
- Identification of adenylate cyclase-stimulating activity and cytochemical glucose-6-phosphate dehydrogenase-stimulating activity in extracts of tumors from patients with humoral hypercalcemia of malignancy.Proceedings of the National Academy of Sciences, 1983