LOWER GASTRIC-SECRETION AND HIGHER INCORPORATION OF OROTIC-ACID INTO LIVER RNA IN RATS TREATED WITH 5-AZACYTIDINE AND CYCLOHEXIMIDE

  • 1 January 1978
    • journal article
    • research article
    • Vol. 25  (3) , 317-327
Abstract
Of the different azapyrimidines tested for their ability to affect metabolism of orotic acid in the liver of rats kept on food, only 5-azacytidine resulted in the enhanced incorporation of orotate into liver RNA following 24 h pretreatment. Similar effect was observed also in cycloheximide-treated animals. No stimulation of orotic acid utilization following 5-azacytidine or cycloheximide [antineoplastic drugs] treatment was observed in the liver of starved animals. Both drugs (but not other pyrimidine analogues tested) markedly depressed gastric secretion in rats and caused decreased evacuation of the stomach. The decreased secretion of pepsin and lower gastric acidity resulting in drug-simulated starvation of the treated animals are discussed in relation to the enhanced uptake of orotic acid into liver RNA.