Endogenous mammalian lectin localized extracellularly in lung elastic fibers

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Abstract
An affinity-purified antibody preparation raised against a .beta.-galactoside-binding lectin from bovine lung was used to localize a similar lectin in rat lung by immunofluorescence and by EM after on-grid staining visualized with colloidal Au conjugated 2nd antibody. The endogenous mammalian lectin was found in smooth muscle cells and squamous alveolar epithelial (type I) cells and was concentrated extracellularly in elastic fibers of pulmonary parenchyma and blood vessels. The extracellular localization of this lectin suggests that it, like others, functions by interaction with extracellular glycoconjugates.