Procedure for rapid isolation of photosynthetic reaction centers using cytochrome c affinity chromatography
- 1 February 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (3) , 683-686
- https://doi.org/10.1073/pnas.80.3.683
Abstract
Horse heart cytochrome c linked to Sepharose 4B is used to purify reaction centers from Rhodopseudomonas sphaeroides R-26. This procedure allows for an initial recovery of 80-90% of the bacterial reaction centers present in chromatophore membranes. High purity reaction centers ( A 280 / A 802 < 1.30) can be obtained with a 30% recovery. Reaction centers from wild-type Rps. sphaeroides and Rps. capsulata also bind to a cytochrome c column. Cytochrome c affinity chromatography can also be used to isolate photosystem I complexes from spinach chloroplasts.Keywords
This publication has 12 references indexed in Scilit:
- A rapid procedure for the isolation and purification of photosynthetic reaction centers from Rhodopseudomonas sphaeroides R-26Archives of Biochemistry and Biophysics, 1982
- Interaction of cytochrome c with reaction centers of Rhodopseudomonas sphaeroides R-26: determination of number of binding sites and dissociation constants by equilibrium dialysisBiochemistry, 1980
- Further studies on the composition and spectral properties of the photochemical reaction centers of bacteriochlorophyll b-containing bacteriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1980
- Effect of detergents on the reliability of a chemical assay for P-700Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1980
- [13] Separation of right-side-out and inside-out submitochondrial particles by affinity chromatography on sepharose-cytochrome cPublished by Elsevier ,1979
- Membrane-bound iron-sulfur centers in photosynthetic systemsBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1978
- Use of immobilized light-harvesting chlorophyll a/b protein to study the stoichiometry of its self-associationBiochemistry, 1978
- Characterization of reaction centers from photosynthetic bacteria. I. Subunit structure of the protein mediating the primary photochemistry in Rhodopseudomonas spheroides R-26Biochemistry, 1974
- Kinetic studies of pigment synthesis by non‐sulfur purple bacteriaJournal of Cellular and Comparative Physiology, 1957
- COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARISPlant Physiology, 1949