Stereospecific opiate binding in living human polymorphonuclear leucocytes
- 1 November 1985
- journal article
- research article
- Published by Wiley in Cell Biology International Reports
- Vol. 9 (11) , 1041-1047
- https://doi.org/10.1016/0309-1651(85)90071-2
Abstract
Living human polymorphonuclear leukocytes were incubated with various opiate agonists and antagonists in radioreceptor assays. Binding of the opiate antagonists 3H-naloxone and 3H-diprenorphine and of the benzomorphan 3H-ethylketocyclazocine was found at 4.degree.C and at 37.degree.C, 3H-naloxone binding was stereospecific. Binding of the opiate agonist 3H-dihydromorphine was present at 37.degree. C but not at 4.degree. C and had a different time course as compared to the antagonists. At both temperatures no specific binding of the proteolytic stable analogue 3H-D-Ala-D-Leu-enkephalin was found. Autoradiography showed an unspecific accumulation of 3H-naloxone inside the cells and a specific localization of grains at the cell membrane.This publication has 3 references indexed in Scilit:
- Opiate receptors on lymphocytes and platelets in manClinical Immunology and Immunopathology, 1983
- Enkephalins Modify Granulocyte-Endothelial Interactions by Stimulating Prostacyclin ProductionThrombosis and Haemostasis, 1983
- Stereoselective muscarinic acetylcholine and opiate receptors in human phagocytic leukocytesBiochemical Pharmacology, 1980