A Radioimmunological Method for Oestrone

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Abstract
A specific antiserum to oestrone1 has been used to develop a radioimmunoassay. No chromatographic separation step was included. A small amount of plasma (0.01–0.15 ml) was extracted once with diethyl ether. the method was used to estimate plasma levels of non‐conjugated oestrone in women during uncomplicated late pregnancy. the oestrone plasma concentrations showed a gradual increase from a mean of 4 ng/ml in the 22nd week to 11 ng/ml in the 41st week. Great individual variations were found. No systematic changes of unconjugated oestrone plasma concentrations were noticed during a 24‐hour period in late pregnancy. Short term studies revealed that large and rapid fluctuations may appear in the levels of unconjugated oestrone during pregnancy.