Temperature-dependent X-ray diffraction as a probe of protein structural dynamics

Abstract
X-ray diffraction at 4 temperatures from 220-300 K coupled with crystallographic refinement yields the mean-square displacements and conformational potentials of all 1261 non-hydrogen atoms of metmyoglobin. Results indicate a condensed core around the heme, semi-liquid regions towards the outside and a possible pathway for ligands. X-ray diffraction can provide the spatial distribution of the dynamic features of a protein.

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