Isolation of an inhibitor of 25-hydroxyvitamin D3-1-hydroxylase from rat serum
- 1 May 1976
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 15 (10) , 2130-2135
- https://doi.org/10.1021/bi00655a016
Abstract
An inhibitor of chick kidney mitochondrial 25-hydroxyvitamin D3-1-hydroxylase was isolated from rat serum by ammonium sulfate precipitation, gel filtration, ion-exchange chromatography and preparative polyacrylamide disc gel electrophoresis. The purified protein contains Fe and has a MW of 52,000. The protein is indistinguishable on gel electrophoresis from a similar inhibitor found in rat kidney tissue. The physiological significance of the inhibitor is not known; however, it may be responsible for the failure to demonstrate in vitro 25-hydroxyvitamin D3-1-hydroxylation with rat and other mammalian tissues.This publication has 4 references indexed in Scilit:
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