POSITIVE SELECTION OF Tac‐ (CD25) POSITIVE CELLS FOLLOWING T‐CELL ACTIVATION: USE OF IMMUNOMAGNETIC SEPARATION AND IMPLICATIONS FOR T‐CELL CLONING

Publisher Website
Google Scholar
Abstract
We have investigated if postive selection for cells expressing activation antigens, which appear on the cell surface during T-lymphocyte activation, could be used for cloning purposes. For this purpose, we used paramagnetic, monodisperse Dynabeads coated with anti-Tac monoclonal antibody, which recognizes CD25 (interleukin-2 receptor light chain). After the first 6-12 h of a primary response, depletion of Tac+ cells could largely abrogate the specific resonse. This indicated that the specifically responding cells were found among the Tac+ population. T-cell cloning was thus performed on Tac+ blasts positively selected after 18 h of a primary response, at day 6 of a primary response or during secodnary stimulation, and gave a high percentage of specific clones. This method is thus a good alternative to establish techniques.