A Mass Spectrometric Solution to the Address Problem of Combinatorial Libraries
- 15 April 1994
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 264 (5157) , 399-402
- https://doi.org/10.1126/science.8153627
Abstract
The molecular weights of femtomole quantities of small peptides attached to polystyrene beads have been determined with imaging time-of-flight secondary ion mass spectrometry. The analysis is made possible by the selective clipping of the bond linking the peptide to a bead with trifluoroacetic acid vapor before the secondary ion mass spectrometry assay. The approach can be applied to large numbers of 30- to 60-micrometer polystyrene beads for the direct characterization of massive combinatorial libraries.Keywords
This publication has 23 references indexed in Scilit:
- Hydrogen Abstraction Reactions in the Kiloelectronvolt Particle Bombardment of Organic FilmsJournal of the American Chemical Society, 1994
- Protein Ladder SequencingScience, 1993
- Ion beams and laser postionization for molecule-specific imagingAnalytical Chemistry, 1993
- Static secondary ion mass spectrometry of adsorbed proteinsAnalytical Chemistry, 1993
- Surface studies by static secondary ion mass spectrometry: cluster ion formation studied by tandem mass-spectrometric techniquesJournal of the Chemical Society, Faraday Transactions, 1992
- A new type of synthetic peptide library for identifying ligand-binding activityNature, 1991
- Generation and use of synthetic peptide combinatorial libraries for basic research and drug discoveryNature, 1991
- SSIMS and SIMS imaging analysis of a drug delivery systemSurface and Interface Analysis, 1988
- Mass spectrometric determination of the amino acid sequence of peptides and proteinsMass Spectrometry Reviews, 1987
- A time-of-flight mass spectrometer for static SIMS applicationsJournal of Vacuum Science & Technology A, 1985