Surface properties of non‐tumorigenic variants of mouse mammary carcinoma cells in culture

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Abstract
The surface properties of two cell lines, Cl 1‐82 and Cl M‐6, from a cultured mouse mammary tumor cell line, FM3A/B, were studied with phytohemagglutinins and the immune adherence test. The transplantabilities of these cells had greatly decreased either spontaneously or after treatment with 4‐nitroquinoline 1‐oxide. The agglutinability of these non‐malignant (low‐ or non‐tumorigenic) cells with agglutinins from various plants was markedly higher than that of the original malignant (tumorigenic) cells. The agglutination was inhibited specifically by certain kinds of sugars. The localization of the receptors of the agglutinin on the cell surface was observed with fluorescent agglutinin.Surface antigens were detected by the immune adherence test with anti‐Cl 1‐82 cells, and their content was found to be higher on non‐malignant than on malignant cells. The increased content was shown to be responsible for induction of strong immunity against malignant cells.