A METHOD FOR THE DETERMINATION OF 17-HYDROXYCORTICOSTEROIDS IN BLOOD: 17-HYDROXYCORTICOSTERONE IN THE PERIPHERAL CIRCULATION*
- 1 May 1952
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 12 (5) , 519-526
- https://doi.org/10.1210/jcem-12-5-519
Abstract
A method is described for the quantitative determination of adrenal steroids in peripheral blood. The method consists essentially of ether-chloroform extraction, partitioning of the ether-chloroform soluble material between 70% ethanol and hexane, and chromatography of the ethanol-soluble fraction on a magnesium silicate-Celite or Florosil column. Quantitative determination of the 17-hydroxycortico-steroids is carried out by the use of a micro modification of the color reaction described by Porter and Silber. Normal values for 17-hydroxycorticosteroids in human peripheral blood ranged between 4 and 10 [mu]g./100 ml. of whole blood.Keywords
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