Growth of a rat neuroblastoma cell line in serum-free supplemented medium.
- 1 January 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (1) , 514-517
- https://doi.org/10.1073/pnas.76.1.514
Abstract
The rat neuroblastoma B104 cell line, which originated in the CNS, was able to proliferate in the absence of serum in synthetic medium supplemented with insulin, transferrin, progesterone, Se and putrescine. When added individually, each supplement had little or no effect; in combination there was a marked synergistic effect on cell number. The cells attained the same saturation density in this medium as in medium with 10% fetal calf serum added. More extensive process formation was observed in the supplemented medium and other differentiated properties were retained as well.This publication has 31 references indexed in Scilit:
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