Gene-product relationships of the nif regulon of Klebsiella pneumoniae

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Abstract
Products of nifV, M, A and F of K. pneumoniae were identified, and suggestive evidence exists for the assignment of products for nifS, U and L. These assignments confirm previous descriptions of transcriptional polarity and suggest the existence of several more nif genes whose products are apparently not essential for N2 fixation under standard laboratory conditions of growth. Although the nifA product is essential for expression of other nif transcripts, it is not involved in the regulation of its own transcript, nifRLA. The stability of nif-encoded proteins under anaerobic conditions was examined in a variety of mutants, confirming previously reported protein-protein interactions and suggesting several new ones. The stability of nif-encoded proteins in NH3 was comparable to that in NH3-free conditions, whereas all nif-gene products appeared to be labile in the presence of O2.