Purification and properties of ornithine aminotransferase from rat brain
- 1 April 1984
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 40 (4) , 357-359
- https://doi.org/10.1007/bf01952550
Abstract
Ornithine aminotransferase (E.C. 2.6.1.13) from rat brain was purified 100-fold by ammonium sulphate fractionation, DEAE cellulose chromatography, calcium phosphate gel and alumina C γ gel. Pyridoxal phosphate was essential for maximum activity of the enzyme. The brain enzyme did not differ from liver and kidney enzymes in properties such as pH optimum, Km, substrate specificity and the inhibition by branched chain amino acids. Unlike rat liver enzyme, brain ornithine aminotransferase was able to catalyze the reaction between L-lysine and 2-oxoglutarate. Spermidine and spermine inhibited brain ornithine aminotransferase activity.Keywords
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