Rapid Screening Assay for Beta-Lactam Antibiotics in Milk: Collaborative Study
- 1 September 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 65 (5) , 1186-1192
- https://doi.org/10.1093/jaoac/65.5.1186
Abstract
A 15 min assay for beta-lactam antibiotics has been used by dairies for several years as a screening procedure for testing milk tankers before they unload. The test is based on a competition between 14C-penicillin and beta-lactam antibiotics in the milk samples for sites on a microbial cell wall that specifically binds beta-lactam. In a collaborative study, 11 laboratories correctly distinguished 10 coded zero penicillin G samples and 10 coded 0.01 IU/mL samples. The proposed test is qualitative, positive or negative, and can detect the presence of beta-lactam antibiotics at the 0.01 IU/mL level. The control point for determining positive or negative samples is more than 3 standard deviations from the mean of 0.01 IU/mL. The method has been adopted official first action.This publication has 1 reference indexed in Scilit:
- Bacillus stearothermophilus Disk Assay for Detection of Residual Penicillins in Milk: Collaborative StudyJournal of AOAC INTERNATIONAL, 1979