ULTRASTRUCTURAL AND MORPHOMETRIC STUDIES OF PLATELETS FROM CATTLE WITH CHEDIAK-HIGASHI-SYNDROME

  • 1 January 1976
    • journal article
    • research article
    • Vol. 35  (3) , 197-204
Abstract
The lesions in platelets from cattle with the Chediak-Higashi syndrome (CHS) are not morphologically analogous to those present in the other granule-containing cells in cattle and other species with CHS. There was no evidence of enlargement of the lysosomal .alpha. granules in bovine CHS platelets. There were decreased numbers of nonlysosomal osmiophilic dense bodies in the bovine CHS platelets. There were also decreased numbers of cytoplasmic vesicles in the CHS platelets. In addition, incubation of the bovine CHS platelets with serotonin did not cause an increase in the number of osmiophilic dense bodies; normal bovine platelets incubated with serotonin had a 3-fold increase in the number of dense bodies. The lesions observed provided a morphologic basis for the bleeding tendency associated with CHS. The term "lysosomal disease" had been applied to CHS and in many types of cells there are morphologic alterations of lysosomes. There apparently is an absence of morphologic alterations of platelet lysosomal granules in bovine CHS platelets and a quantitative deficit of the platelet nonlysosomal granules in these cells. Not all lysosomes in CHS are abnormal and some of the abnormal granules in CHS are not lysosomes.