Sulfated Oligosaccharides From K-Carrageenan and Oligogalacturonic Acids Separation By H.P.L.C.

Abstract
Sulfated oligosaccharides produced from enzymic hydrolysis of K-carrageenan have been separated up to DP 13 on a column of μ-Bondapak C-18 or a Radial Pak cartridge “Dextropak” with sodium nitrate as the mobile phase. Moreover, the separation of oligogalacturonic acids has been performed by the ion pair method on the same supports with 0.05% dodecyltributylammonium chloride in a sodium nitrate solution as eluent.