Tissue Culture of Bulb Scale Sections for Asexual Propagation of Lilium longiflorum Thunb.1

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Abstract
An average Easter lily bulb with 100 scales may produce 8000 or more bulbs in 6 weeks when 1 mm thick cross sections from the scales are cultured in the Linsmaier-Skoog medium as modified by Sheridan and supplemented with 0.03 mg/liter NAA. Continuous darkness at 25°C increased bulb number and size at the expense of leaf number and size while a cycle of 16 hours cool white fluorescence at 1.6 klx (150 ft-c) and 8 hours dark at 25°C suppressed bulb formation but enhanced leaf formation, root weight, and fresh weight of callus. Root numbers were equal in both environments. Cultures incubated at 18.3° exhibited no measurable growth after 6 weeks. Explants from the distal part of the bulb scale will grow only with growth regulators present.

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