PRE3, highly homologous to the human major histocompatibility complex‐linked LMP2 (RING12) gene, codes for a yeast proteasome subunit necessary for the peptidylglutamyl‐peptide hydrolyzing activity
Open Access
- 21 March 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 341 (2-3) , 193-196
- https://doi.org/10.1016/0014-5793(94)80455-9
Abstract
20S proteasomes are multifunctional proteinase complexes ubiquitous in eucaryotes. We have cloned the yeast PRE3 gene by complementation of the pre3‐2 mutation, which leads to a defect in the peptidylglutamyl‐peptide hydrolyzing activity of the 20S proteasome. The PRE3 gene, a β‐type member of the proteasomal gene family, is essential for cellular life and codes for a 193‐amino acid proteasomal subunit with a predicted molecular mass of 21.2 kDa. The Pre3 protein shows striking homology to the human proteasome subunits Hsδ and Lmp2 (Ring12). Lmp2 is encoded in the major histocompatibility complex class II region implicating proteasomes in antigen processing.Keywords
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