A Method for Demonstrating the Presence of Alkaline Phosphatase and Glycogen in the same Section

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Abstract
Tissue is fixed in absolute alcohol and embedded in paraffin. The sections are treated by Kabat and Furth''s modification (1941) of Gomori''s technic for demonstrating alkaline phosphatase as far as the stage of treatment with ammonium sulfide. After removal of the sections from the ammonium sulfide they are quickly rinsed in water, drained well and treated with freshly prepd. Best''s carmine for 10 min. The sections are differentiated in Best''s differentiating fluid, transferred to equal parts of ether and absolute alcohol to re-move the celloidin film, rinsed in absolute alcohol, cleared in xylene, and mounted in balsam.