AUTOMATED CREATINE KINASE-MB ESTIMATION BY IMMUNO-INHIBITION - A CLINICAL-EVALUATION

  • 1 January 1980
    • journal article
    • research article
    • Vol. 26  (5) , 568-572
Abstract
The presence of creatine kinase (EC 2.7.3.2) MB isoenzyme (CK-MB) was detected in serum by using an immunoinhibition technique to inactivate the creatine kinase isoenzyme of muscle origin (CK-MM). The GEMSAEC centrifugal analyzer rapidly mixes reagents and has a throughput of 60 samples/h. The within-run coefficient of variation was acceptable when the CK-MB value was 7.5 U/l or more. CK-MM was totally inactivated up to 5000 U/l, a value above that usually seen in myocardial-infarct patients. The predictability of the assay for detecting myocardial infarction was assessed among 120 coronary-care patients when 2% of total CK activity was considered diagnostic and the results were compared with those obtained with an electrophoresis technique. The positive predictive value of the immunoinhibition assay was 97.8%, compared to 92% with the CK electrophoresis technique, when lactate dehydrogenase (EC 1.1.1.27) isoenzyme results were included in both cases. The optimal negative predictive value was 95.1% with the immunoinhibition assay vs. 98.2% with electrophoresis. The immunoinhibition technique for estimating CK-MB can be automated to assess myocardial status rapidly, precisely, inexpensively and sensitively.

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