Movement of Aldolase From Excised Rat Diaphragm

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Abstract
A protein, aldolase, flows from excised rat diaphragm incubated in a variety of media. The rate of outflow of aldolase is increased by anoxia and by a high potassium concentration in the medium, and it is decreased by a reduction in temperature and by addition of glucose. When diaphragm is transferred to fresh media and reincubated the rate of outflow of aldolase is also accelerated. From measurements of rates of outflow of aldolase, estimates have been made of the area of the membrane required for aldolase to flow through the cell membrane as though it were flowing simply through an aqueous solution. This area is about 10–7–10–8 of the estimated total fiber surface. The estimated area for diffusion of aldolase is modified 16-fold by factors which alter the metabolism of diaphragm.

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