Bone Cells: A Serum-Free Medium Supports Proliferation in Primary Culture
- 3 February 1978
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 199 (4328) , 542-544
- https://doi.org/10.1126/science.564080
Abstract
Bone-cells isolated from embryonic rat calvaria increase in number two-to threefold when cultured at high, but not at low, population densities in a serum-free medium that contains albumin. Cultured cells respond to parathyroid hormone and exhibit a marked rise in alkaline phosphatase activity during proliferation, which suggests the progressive differentiation or preferential growth of osteoblast-like cells.Keywords
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