Calcium Channel Antagonists Verapamil and Gallopamil Are Powerful Inhibitors of Acid Secretion in Isolated and Enriched Guinea Pig Parietal Cells
- 1 January 1983
- journal article
- research article
- Published by S. Karger AG in Pharmacology
- Vol. 27 (1) , 9-14
- https://doi.org/10.1159/000137824
Abstract
In isolated and enriched guinea pig parietal cells the inhibitory effects of the calcium channel antagonists verapamil and gallopamil on 14C-aminopyrine uptake (= H+ secretion) have been analyzed. Both verapamil and gallopamil inhibit acid secretion in a concentration-dependent manner with an IC50 of 12.1 and 10.9 μmol/l respectively. The type of inhibition is noncompetitive in nature. Verapamil inhibits the acid respone to histamine, dibutyryl-cAMP, and KC1 with IC50 values not significantly different from each other. Exposure of the cells to verapamil and subsequent washing enhances the acid response to histamine for an unknown reason. It is concluded that the calcium channel antagonists verapamil and gallopamil inhibit acid secretion in vitro by interfering with the parietal cell proton pump, the K+/H+-ATPase.Keywords
This publication has 2 references indexed in Scilit:
- Extracellular calcium and cholinergic stimulation of isolated canine parietal cells.Journal of Clinical Investigation, 1981
- The Actions of Secretagogues on Oxygen Uptake by Isolated Mammalian Parietal CellsJournal of Clinical Investigation, 1978