Countercurrent Separation: A New Method for Studying Behavior of Small Aquatic Organisms
- 1 May 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (5) , 1349-1352
- https://doi.org/10.1073/pnas.70.5.1349
Abstract
A new method for the analysis of behavior in small free-swimming aquatic organisms is described. In this procedure, called countercurrent separation, a dense solution flows down along the bottom of an inclined chamber while a light solution flows in the opposite direction, upward along the top of the chamber. The attraction of animals (injected into the center of the chamber) to one solution or the other is then determined by observing the proportion of animals that emerges from the chamber in that solution. When used with the nematode Caenorhabditis elegans, it is estimated that the apparatus is equivalent to at least nine theoretical plates.Keywords
This publication has 4 references indexed in Scilit:
- Isolation of different classes of spinach chloroplasts by counter-current distribution: A methodological studyBiochimica et Biophysica Acta (BBA) - Biomembranes, 1972
- From the gene to behavior.1971
- BEHAVIORAL MUTANTS OF Drosophila ISOLATED BY COUNTERCURRENT DISTRIBUTIONProceedings of the National Academy of Sciences, 1967
- Separation of Bacteria by Counter-Current DistributionNature, 1961