Computation of response factors for quantitative analysis of monoterpenes by gas-liquid chromatography

Abstract
Response factors for quantitative analysis by gas-liquid chromatography were computed for 10 monoterpenes usingp-cymene as an internal standard. The results show that there is no significant variation in our estimates due to sample concentration, and very low variation due to the compound. The most significant deviation fromRF c=1.0 occurred with myrcene. We conclude that the underlying assumptions of quantitative analysis are met usingp-cymene as an internal standard for most plant or animal monoterpenes, and for total monoterpene content.