Chromatography and Biological Stains: I. Paper Chromatography as a Means of Determining Purity and Dye Content of Sudans III and IV. 1

Publisher Website
Google Scholar
Abstract
By paper chromatographic technics, using iso-octane as the solvent, we have been able to analyze commercial Sudans III and IV for the numerous colored components present. This tech-nic is invaluable in the qualitative analysis of the dyes studied, and, in addition, a quantitative estimation can be accomplished by a serial dilution method described. It would appear that there is a difference between the Sudan III or Sudan IV concentration found by this method and the total dye content found by the chemical reduction method. A detailed description of the procedure and a discussion of the results are included.
Keywords