Development and Use of Radioimmunoassays for the Detection of Human IgG Antibodies to Laboratory Animal Urinary Proteins
- 1 January 1982
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 69 (4) , 363-367
- https://doi.org/10.1159/000233201
Abstract
A radioimmunoassay method for the detection of human IgG antibodies to laboratory animal urinary proteins has been developed using partially purified protein fractions prepared from crude urine extracts by gel filtration. The proteins from the four species, mouse, rat, guinea pig and rabbit, were labelled with 125I and used in an antigen binding assay employing a sepharose-linked staphylococcal protein A imunosorbent. Results, expressed as arbitrary units of specific IgG per unit serum, show antibodies to one or more species present in 57 % of current animal workers compared with 6 % of non-animal workers.This publication has 1 reference indexed in Scilit:
- Laboratory animal allergy: a clinical survey of an exposed population.Occupational and Environmental Medicine, 1981