The Influence of Metabolic Inhibitors and Incubation Time on Aflatoxin Release from Aspergillus flavus

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Abstract
Four metabolic inhibitors were tested for their ability to interfere with aflatoxin release at concentrations which did not inhibit growth of A. flavus in synthetic media as indicated by mycelial dry weight determinations. The amount of aflatoxin B1 released in cultures incubated with either N,N-dicyclohexylcarbodiimide or sodium azide was markedly lower than in control cultures. Toxin release was inhibited to a smaller degree by potassium cyanide and 2,4-dinitrophenol at the levels tested. Presence of the latter 2 inhibitors resulted in a delay of time when peak levels of toxin were released into media. Mycelial extracts from cultures incubated in the presence of N,N-dicyclohexylcarbodiimide contained higher amounts of aflatoxin than did extracts from controls. An energy-dependent transport system is probably involved in the release of aflatoxins from A. flavus.

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