Early synthesis of specific cytoplasm proteins is correlated with the rate of exit of lymphocytes from the resting state.

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Abstract
The initiation of synthesis of proteins in human lymphocytes exposed to the mitogen phytohemagglutinin (PHA) for 6 h was investigated. Radiolabeled proteins in 3 subcellular fractions, cytoplasmic, nuclear salt wash and nuclear, were separated on polyacrylamide gels. Compared with cells incubated for the same time in the absence of PHA only 2 cytoplasmic proteins of MW 51 and 60 kD [kilodalton] showed increased synthesis in a dose-dependent fashion. Synthesis of the 60 kD protein shows the strongest correlation with rate of entry into the first S phase and with rate of cellular aggregation. The 60 kD protein appears to be a major early response-associated protein for entry of lymphocytes into the 1st S phase after PHA stimulation.