Purpald (4-Amino-3-hydrazino-5-mercapto-1, 2, 4-triazole) as a Reagent for Post-Column Derivatization of Neutral Monosaccharides in High Pressure Liquid Chromatography
- 1 April 1993
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 16 (5) , 1105-1116
- https://doi.org/10.1080/10826079308019574
Abstract
A comprehensive study of the reaction between Purpald (4-amino-3- hydrazino-5-mercapto-l, 2, 4-triazole) and eight neutral monosaccharides in order to establish the optimal conditions (wavelength, oxygen supply, temperature, time, pH and concentration range) was carried out. The reaction was applied to the post-column detection in HPLC of monosaccharides eluted with water from an ion-exchange column. The detection limits and linear determination ranges thus achieved make this reaction a valid alternative to the determination of carbohydrates.Keywords
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