The Relationship of Temperature and Nutrition to the Growth of Sunflower Tumor Tissue in Vitro.

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Abstract
The growth of sunflower stem tumor tissue and habituated sunflower stem tissue on several media was observed. Tumor tissue growth was found to be more retarded at 33-34oC on medium lacking supplements (casein hydrolysate, adenine sulfate, an 11-vitamin mixture, indoleacetic acid) than when they were grown at this temperature on medium containing supplements. The autonomous nature of tumor tissue is altered at temperatures above the normal for growth. The presence of supplements in the medium offers a partial replacement for a naturally occuring factor in tumor tissue which is wholly or partly responsible for its autonomous nature. The factor, TPP, (tumor progression principal) is assumed to be similar or analogous to the tumor induction principal, TIP. IAA is essential to the recovery of growth of the tissue. Tumor tissue growth after thermal treatment becomes similar to the growth of habituated tissue grown on the basal medium, minus supplements and auxin. Habituated tissues are unaffected by the temperature change when grown on the complete medium, salts, sugar, casein hydrolysate, adenine sulfate, and a mixture of vitamins. (GBA) plus IAA.

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