Cryopreservation of Complement‐Coated Erythrocytes

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Abstract
Cells coated with complement components (C3b-C4b and C4b cells) were prepared by various methods and stored in liquid N using a low-glycerol, rapid-freeze technique. Freshly coated cells and cells coated and then frozen were tested vs. various antiglobulin and anticomplement reagents to evaluate the reactivity of such frozen, stored, complement-coated human red blood cells. Liquid N preservation of such coated cells proved to be feasible when such cells were compared with freshly coated control cells.