Simultaneous Determination of Verapamil and Celiprolol in Human Plasma
- 1 April 1994
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 32 (4) , 153-156
- https://doi.org/10.1093/chromsci/32.4.153
Abstract
A simple and reproducible method for the simultaneous determination of the β1-selective adrenergic blocker, celiprolol, and the calcium antagonist, verapamil, in human plasma is described. It involves a two-step liquid—liquid extraction and separation using a C18 column with ultraviolet detection at 237 nm. Deacetyldiltiazem is used as the internal standard. Within-day and between-day coefficients of variation are less than 10%. The lower limits of detection are 4, 2, and 4 ng/mL for celiprolol, deacetyldiltiazem, and verapamil, respectively. The assay has clinical applicability.Keywords
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