Vaccinia Virus Recombinant Expressing an 87-Kilodalton Polyprotein That Is Sufficient To Form Astrovirus-Like Particles
Open Access
- 15 August 2003
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (16) , 9094-9098
- https://doi.org/10.1128/jvi.77.16.9094-9098.2003
Abstract
Human astrovirus is an important cause of acute gastroenteritis. We have generated, for the first time, a vaccinia virus recombinant expressing the astrovirus 87-kDa structural polyprotein. The results demonstrate that this expression results in the formation of virus-like particles in the absence of other astrovirus proteins and genomic RNA. The purified trypsin-activated virus-like particles strongly resemble the complete astrovirus particles.Keywords
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