Synthesis of γ-Phosphate-Linked Nucleoside Affinity Chromatography Resins for Protein Purification, Including Ribonucleoside Triphosphate Reductase
- 1 August 1998
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 17 (8) , 1453-1471
- https://doi.org/10.1080/07328319808003480
Abstract
Seven nucleotides linked through the γ-phosphate to diamine hydrocarbons were synthesized and coupled to Sepharose for use in protein purification affinity chromatography. The synthesis involved converting the nucleotides to nucleoside-5′-trimetaphosphates using dicyclohexyl carbodiimide, followed by nucleophilic ring opening of the trimetaphosphate with an α,ω-diamino hydrocarbon to generate a γ-phosphoamide linkage in each nucleotide.Keywords
This publication has 38 references indexed in Scilit:
- Thiyl Radicals in Ribonucleotide ReductasesScience, 1996
- Electron Paramagnetic Resonance Investigations of a Kinetically Competent Intermediate Formed in Ribonucleotide Reduction: Evidence for a Thiyl Radical-Cob(II)alamin InteractionJournal of the American Chemical Society, 1996
- Coenzyme B12-Dependent Ribonucleotide Reductase: Evidence for the Participation of Five Cysteine Residues in Ribonucleotide ReductionBiochemistry, 1994
- Cloning, sequencing, and expression of the adenosylcobalamin-dependent ribonucleotide reductase from Lactobacillus leichmannii.Proceedings of the National Academy of Sciences, 1993
- Adocobalamin (AdoCbl or coenzyme B12) cobalt-carbon bond homolysis radical-cage effects: product, kinetic, mechanistic, and cage efficiency factor (Fc) studies, plus the possibility that coenzyme B12-dependent enzymes function as "ultimate radical cages" and "ultimate radical traps"Inorganic Chemistry, 1993
- Radical cage effects in adocobinamide (axial-base-off coenzyme B12): a simple method for trapping [Ado.bul. .bul.CoII] radical pairs. A new .beta.-H elimination product from the radical pair and measurement of an unprecedentedly large cage-recombination efficiency factor, Fc .gtoreq. 0.94Journal of the American Chemical Society, 1992
- Mechanism of inactivation of Escherichia coli and Lactobacillus leichmannii ribonucleotide reductases by 2'-chloro-2'-deoxynucleotides: evidence for generation of 2-methylene-3(2H)-furanoneBiochemistry, 1984
- Thermolysis of adenosylcobalamin: a product, kinetic, and cobalt-carbon (C5') bond dissociation energy studyInorganic Chemistry, 1984
- Substrate and effector binding to ribonucleoside triphosphate reductase of Lactobacillus leichmanniiBiochemistry, 1974
- Cobamides and Ribonucleotide ReductionPublished by Elsevier ,1965