Evidence that 16S RNA from E.coli can assume two different biologically active conformations
Open Access
- 1 August 1976
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (8) , 1883-1902
- https://doi.org/10.1093/nar/3.8.1883
Abstract
We have recently shown1 that 16S RNA can be extracted from 30S ribosomes by an acetic acid - ureaprecipitation procedure which yields RNA capable of binding 13 individual ribosomal proteins. This is in contrast to phenol extracted 16S RNA which cans pecifically associate with only 7 proteins2–7. in the experiments reported here, we demonstrate that the difference in protein binding capacities is due to a relatively more “open” configuration possessed by the aceticacid-urea 16S RNA.Keywords
This publication has 19 references indexed in Scilit:
- Detection of cation-specific conformational changes in ribosomal RNA by gel electrophoresisNucleic Acids Research, 1974
- Radioactive Labeling of Proteins in VitroJournal of Biological Chemistry, 1971
- Three-Dimensional Organization of the 30S Ribosomal Proteins from Escherichia coli , I. Preliminary Classification of the ProteinsProceedings of the National Academy of Sciences, 1970
- Nucleotide Sequences of Sections of 16S Ribosomal RNANature, 1970
- Assembly Mapping of 30S Ribosomal Proteins from E. coliNature, 1970
- Ribosomal proteins of Escherichia coli. I. Purification of the 30 S ribosomal proteinsBiochemistry, 1969
- Structure and function of Escherichia coli ribosomes: VI. Mechanism of assembly of 30 s ribosomes studied in vitroJournal of Molecular Biology, 1969
- Analytical studies on nuclear ribonucleic acid using polyacrylamide gel electrophoresisBiochemistry, 1968
- The secondary structure of ribosomal ribonucleic acid in solutionBiochemical Journal, 1966
- A two-dimensional fractionation procedure for radioactive nucleotidesJournal of Molecular Biology, 1965