IMMUNOLOGICAL ABNORMALITIES IN AN ANIMAL-MODEL OF CHRONIC HYPOPLASTIC MARROW FAILURE INDUCED BY BUSULFAN

  • 1 January 1978
    • journal article
    • research article
    • Vol. 51  (4) , 601-610
Abstract
The immunology of chronic hypoplastic marrow failure (CHMF, aplastic anemia) was studied in an experimental murine model of the disease induced by busulfan. B [bone marrow-derived] lymphocytes of peripheral blood, spleen and bone marrow were reduced to 30%-40% and T [thymus-derived] lymphocytes of thymus, spleen, marrow and blood were decreased to 20%-70% of control values. Ig[immunoglobulin]G and IgM antibody titers to sheep red blood cells were reduced to 1/3 of control levels, and splenic IgG, but not IgM, plaque-forming cells were fewer on day 7 after antigen stimulation. The proliferative responses to phytohemagglutinin or concanavalin A were reduced in cultures of peripheral blood lymphocytes, splenic lymphocytes and thymocytes, and cutaneous delayed-type hypersensitivity induced by dinitrofluorobenzene was not detected in mice with CHMF. The results demonstrate disturbance of a vareity of cellular and humoral functions and suggest that the disturbance was due to quantitative and possibly qualitative abnormalities of the cell types subserving these functions. Residual cell injury, the lesion underlying experimental CHMF, is probably not confined to the myeloid stem cell but also involved cells of the lymphoid series.

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