ATP-driven Ca2+/H+ antiport in acid vesicles from Dictyostelium.
Open Access
- 1 September 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (17) , 8025-8029
- https://doi.org/10.1073/pnas.89.17.8025
Abstract
Amoebae of the cellular slime mold Dictyostelium discoideum possess an extensive and dynamic endomembrane system that includes many types of acidic vacuoles. A light membrane fraction from Dictyostelium, rich in vacuolar-type H(+)-ATPase, has been described [Padh, H., Lavasa, M. & Steck, T.L. (1989) J. Cell Biol. 108, 865-874]. Here, we show that this "acidosomal" fraction also contains a high-affinity vanadate-sensitive Ca2+ uptake activity that is stimulated by the pH gradient formed by the H(+)-ATPase. We attribute this Ca2+ uptake to the presence of a H(+)-countertransporting Ca(2+)-ATPase, pumping Ca2+ into an acidic compartment.Keywords
This publication has 28 references indexed in Scilit:
- Calcium homeostasis in Trypanosoma brucei. Identification of a pH-sensitive non-mitochondrial calcium pool.1991
- Different calcium pools in human platelets and their role in thromboxane A2 formation.Journal of Biological Chemistry, 1991
- Identification of a high-affinity Ca2+ pump associated with endocytotic vesicles in Dictyostelium discoideumExperimental Cell Research, 1989
- Characterization of a vacuolar proton ATPase in Dictyostelium discoideumBiochimica et Biophysica Acta (BBA) - Biomembranes, 1989
- H+-dependent calcium uptake into an IP3-sensitive calcium pool from rat parotid glandAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 1988
- Inositol 1,4,5-trisphosphate induces calcium release from a non-mitochondrial pool in amoebae of DictyosteliumBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
- Calcium transport driven by a proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae.Journal of Biological Chemistry, 1983
- Intracellular pH and the control of cell differentiation in Dictyostelium discoideumNature, 1983
- Characterization of the plasma membrane ATPase of Saccharomyces cerevisiaeMolecular and Cellular Biochemistry, 1978
- Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic cultureBiochemical Journal, 1970