High resolution ultrathin‐layer isoelectric focusing of PGM1‐subgroups in forensic blood typing
- 1 February 1981
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 2 (5-6) , 327-330
- https://doi.org/10.1002/elps.1150020514
Abstract
Ultrathin‐layer isoelectric focusing in 100 um polyacrylamide gels is described for the separation of the isozymes of the PGM1‐locus. The PGM1‐subgroups are best resolved in pH 5–7 gradients obtained by mixing Ampholine and Servalyt carrier ampholytes in a 2:1 ratio. By using a high field strength (180 V/cm) in the final stage of isoelectric focusing, the isozyme bands are sharper than in conventional thin‐layer gels. This facilitates identification of the subgroups. Distortions of the pH gradient are avoided if low initial field strengths (15–20 V/cm) are used. The method is highly reproducible and equally applicable for the analysis of blood samples as well as blood stains.This publication has 8 references indexed in Scilit:
- Ultrathin‐layer isoelectric focusing in 50–100 μm polyacrylamide gels on silanized glass plates or polyester filmsElectrophoresis, 1980
- Genetic Evidence for Four Common Alleles at the Phospho‐glucomutase‐1 Locus (PGM1) Detectable by Isoelectric FocusingVox Sanguinis, 1978
- Investigations on the PGM 1 a polymorphism (phosphoglucomutase-EC 2.7.5.1) by isoelectric focusingHuman Genetics, 1978
- Evidence for two additional common alleles at the PGM1 locus (Phosphoglucomutase-E.C.:2.7.5.1)Human Genetics, 1977
- Typing of the Common Phosphoglucomutase Variants Using Isoelectric Focusing—A New Interpretation of the Phosphoglucomutase SystemJournal of the Forensic Science Society, 1976
- A Micro Procedure for Fast Typing of the Genetic Variants of PhosphoglucomutaseJournal of the Forensic Science Society, 1974
- Phosphoglucomutase Polymorphism in ManNature, 1964
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951