A MONOCLONAL IGM SMOOTH-MUSCLE ANTIBODY REACTIVE WITH FIBROBLAST STRESS FIBERS PRODUCED BY IMMUNIZATION WITH TREPONEMA-PALLIDUM

  • 1 January 1983
    • journal article
    • research article
    • Vol. 52  (3) , 537-542
Abstract
A monoclonal IgM smooth muscle antibody secreted by a hybrid (MMI-1) of mouse plasmacytoma NS-1 with spleen cells from mouse immunized with T. pallidum was detected by indirect immunofluorescence tests on frozen tissue sections and on acetone fixed monolayers of rat and human fibroblasts. The antibody did not react with acetone fixed smears of T. pallidum but reacted with smooth muscle fibers and with striations of skeletal and cardiac muscle. In non-muscle cells, the antibody stained liver in a polygonal pattern, thymus and accentuated staining of the thymic medulla, renal glomeruli and the brush border and peritubular fibrils of renal tubules. In fibroblast monolayers, the antibody stained stress fibers in an interrupted pattern. Immunoblotting with muscle proteins and the antibody showed labeling of a 100K molecule. The cellular distribution of the mouse monoclonal antibody is similar to that obtained with anti-actin antibody suggesting that the corresponding antigen may be an actin binding protein.