Direct multicomponent analysis of beer samples constituents using micellar electrokinetic capillary chromatography

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Abstract
A capillary electrophoretic method was developed using micellar electrokinetic capillary chromatography (MEKC) with diode‐array detection to analyze simultaneously 26 beer constituents in a single procedure, including alcohols, iso‐α‐acids, amino acids, flavonoids, isoflavonoids, a vitamin, purine and pyrimidine bases. After filtration, sample components were separated with an uncoated capillary and a 25 mM sodium borate and 110 mM SDS buffer at pH 10.5. Analyses were run at 14 kV and 8 s of hydrodynamic injection with UV detection at 210 nm and 270 nm. The proposed method was successfully applied to the direct determination of beer constituents without any sample cleanup procedures.