Novel Polyomavirus Detected in the Feces of a Chimpanzee by Nested Broad-Spectrum PCR
Open Access
- 15 March 2005
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 79 (6) , 3883-3887
- https://doi.org/10.1128/jvi.79.6.3883-3887.2005
Abstract
In order to screen for new polyomaviruses in samples derived from various animal species, degenerated PCR primer pairs were constructed. By using a nested PCR protocol, the sensitive detection of nine different polyomavirus genomes was demonstrated. The screening of field samples revealed the presence of a new polyomavirus, tentatively designated chimpanzee polyomavirus (ChPyV), in the feces of a juvenile chimpanzee (Pan troglodytes). Analysis of the region encoding the major capsid protein VP1 revealed a unique insertion in the EF loop of the protein and showed that ChPyV is a distinct virus related to the monkey polyomavirus B-lymphotropic polyomavirus and the human polyomavirus JC polyomavirus.Keywords
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